EFFECT OF QUERCETIN SUPPLEMENTATION ON MUSCLE HYPERTROPHY
J.O. BOUVIERE1*, M.R. BALDANZA2, J.P.S WERNECK-DE-CASTRO2 AND A.P.T.R PIERUCCI1
1Universidade Federal do Rio de Janeiro, Instituto de Nutrição Josué de Castro
2Universidade Federal do Rio de Janeiro, Departamento de Biociências e Atividade Física
Quercetin is a bioactive compound belonging to the class of flavonoids with variable structure phenolic presenting considerable scientific and therapeutic interest. The present study aimed to elucidate the effect of quercetin supplementation on hypertrophy muscle of Wistar rats measuring gene expression pathways in muscle hypertrophy through real-time PCR and analyzing the fiber diameter of soleus muscles through the area cross-section. There was the supplementation with quercetin and found promoted hypertrophy of soleus muscles (for histological analysis). Specifically this supplemented with quercetin 25mg/kg for 7 days led to increase the diameter of the soleus muscle fiber in sedentary and exercised animals in accordance with the results of histological analyzes (p=0.0214). The analysis of gene expression profiles was investigated by testing quantitative Real Time PCR. The results indicates that treatment with quercetin led to an increase in the expression of PGC-1a (p=0.0015) and indicates no significant increases in the expression of IGF-1 (0.4334). Taken together our results indicate that treatment with quercetin is capable of promoting hypertrophy soleus muscles of Wistar rats and suggest that the mechanism of increased muscle is independent of IGF-1. Furthermore, it is suggested that the PGC-1a gene and its alternative transcripts may be important molecular targets modulated by quercetin and can contribute to the beneficial effects of muscle mass gain of this flavonoid.
Financial Support: FAPERJ, CAPES E CNPq ; Area: Biochemistry;
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