PREPARATION AND CHARACTERIZATION OF NANOSTRUCTURED ZINC OXIDE FOR INVESTIGATING ITS EFFECT ON THE GASTROINTESTINAL TRACT OF WEANLING PIGLETS
M.S.R. BARRETO1,C.T. ANDRADE2
1Universidade Federal do Rio de Janeiro, Instituto de Química
2Universidade Federal do Rio de Janeiro, Instituto de Macromoléculas
Along last decades, pharmacological doses of zinc oxide have been used to combat post-weaning diarrhea and improve animal performance. The first week post-weaning are particularly stressful for the animals and immunosuppressant. The bacterium Escherichia coli is the most important in this phase of growth and it is believed that ZnO directly inhibits the intestinal environment. Smaller ZnO particles with a greater surface contact with the gastrointestinal medium are expected to favor the effectiveness of the product as a growth promoter. The use of biopolymers in the synthesis of ZnO has been proposed as a strategy to control growth and agglomeration of the nanoparticles. In this study, two samples of ZnO were synthesized. The first was obtained from solutions of zinc di-acetate and sodium hydroxide solution in the presence of sodium alginate at 0.25% concentration and the other solution in the presence of high methoxyl pectin at 0.5% concentration. The samples were characterized by X-ray diffraction (XRD), infrared absorption spectroscopy (FTIR) and scanning electron microscopy (SEM). The difratogramas found for both samples were almost identical as to the position of reflections, which shows that ZnO was successfully synthesized. However, the diffractrograms showed differences in the intensity of the reflections, characteristic associated with the crystallinity of the material. The application of the Scherrer’s equation revealed a value of 15 nm for the average size of the crystal. The FTIR spectra of the samples presented an intense absorption around 500 cm -1, characteristic of ZnO, which confirms the results of XRD. Tests are underway to investigate the antimicrobial effect of samples in vivo. The performance parameters, intestinal microbiology, villus height of the duodenum, crypt depth and villus height ratio / crypt depth will be evaluated.
Financial support: CAPES and Auster Nutrição Animal Ltda.; Area: Food Science
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