CHARACTERIZATION OF SOLUBLE PROTEINS INVOLVED IN CHEMICAL COMMUNICATION OF Rhodnius prolixus
OLIVEIRA, D.S.; ABREU, P.A.; MOREIRA, M.F.; JUNQUEIRA, M.R.; DOMONT, G.; SOARES, M.R.; MELO A.C.A.
Universidade Federal do Rio de Janeiro, Instituto de Química, Departamento de Bioquímica
R. prolixus is the vector of Trypanosoma cruzi, etiological agent of Chagas disease, which affects ca. 7-8 million in American. Green technologies are an alternative to reduce this vector population. Odor decoding by the insects are done by different proteins, including odorant binding proteins (OBPs), and chemossensorial proteins (CSPs). Together with other proteins they participate in coordinating manner to present odor molecules to their receptors in the membrane of the olfactory neuron. Our purpose was to describe a protein map in male (MA) and female (FA) antennae of R. prolixus and identify possible protein targets for control. Proteins from antennae were extracted and analyzed using one-dimensional electrophoresis (1D), two-dimensional electrophoresis (2D) and off-gel assay followed by LC-MS/MS and GelLC-MS/MS on LTQ-Orbitrap and Nano LC-MS/MS analysis. Searches were done with MS/MS profiles using Mascot (http://www.matrixscience.com). A total of 564 proteins were identified in MA and 708 proteins in FA. Those proteins were classified in different cellular functions as, energy metabolism, nervous system/cell signaling, defense and detoxification, protein metabolism, cell motility and chemoreception. Proteins involved directly with olfaction were further identified: 15 OBPs in MA and 16 OBPs in FA, 5 CSPs in MA and 6 CSPs in FA. It was observed 1 OBP and 1 CSP specifically expressed in FA. Rhodnius prolixus’s transcriptome (Ribeiro et al., 2014) predicts 46 candidates for OBPs/CSPs. Our results show that 22 OBPs/CSPs are functionally expressed in antennae. This study describes for the first time, the expression profile of proteins in the antennae of R. prolixus, opening new perspectives for research in the area of chemical communication in insect vectors.
Financial Support: FAPERJ, INCT-EM/CNPq, CAPES; Area: Biochemistry.
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