ASSESSMENT OF ANTIOXIDANT ACTIVITY OF COORDINATION COMPOUNDS IN SACCHAROMYCES CEREVISIAE UNDER OXIDATIVE STRESS
D. D. QUEIROZ1*, HORN A.2 AND M. D. PEREIRA1
1Laboratório de Citotoxicidade e Genotoxicidade, Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Instituto de Química
2Universidade do Norte Fluminense
Oxidative stress has been widely related to several pathologies, including neurodegenerative diseases and cancer. Aiming to reduce the damages and to increase cell tolerance against oxidative stress and its consequences, many studies have been performed to find new antioxidant compounds. One of the recent strategies to reduce oxidative stress is the development of metal complexes with redox activity. Some of these complexes are capable to decompose reactive oxygen species, contributing to redox balance maintenance avoiding cell injuries. Using Saccharomyces cerevisiae as a eukaryotic model, the main goal of this work was to evaluate the ability of six coordination compounds (C1a, C1b, C1c, C2a, C2b and C2c) in protecting cells against the harmful effects of oxidative stress. Cells were previously submitted to complexes treatment before being exposed to oxidative stress and then plated to evaluate cell tolerance. Our results indicate that all complexes were capable protect wild type cells against the menadione – related oxidative stress. However, only C1b, C2a and C2c are capable to promote full protection in superoxide dismutase (SOD) deficient strain stressed with menadione. Taken together our results suggest that these complexes can protect cells against oxidative stress by mimicking the activity of superoxide dismutase. In addition, C1a, C1c and C2b, seems to interact with SOD to provide full cellular protection.
Financial Support: FAPERJ, CAPES E CNPq; Area: Biochemistry.
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